Telomere Analysis - Looking Out for Number One.
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Highlight Links
Telomere Measurements
Telomerase Expression Measurements
Telomere Articles and Links
Cancer and the Gene
Measuring Cellular Senescence
Clinical Testing
Academic Laboratories and Programs [JenAge/Ageing-related Research Centres and Institutes]
Symposia and Conferences in Anti-Aging Medicine [Links, JenAge/Meetings Calendar]
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Telomere Measurements [Refs 7.11, Links, Images, Video, Papers, Patents, Books, Amazon] (See [81s] on telomerase activators for lengthening telomeres.)
__[Dr. Jerry Shay of Shay-Wright Labs speaks about telomere measurements. Also see Wilbur E. Wright on Telomere Biology in Aging and Cancer].
Telomere Length Assays [Links, Images, Video, Papers, Patents, Books, Amazon].
__Commercial Telomere Length Assay Labs:
______SpectraCell ($290.00) [Site, Links, Images, SpectraCell Telomere Results (T-Lymphocytes only) (Images)] spec1@spectracell.com,
___________Today SpectraCell measurements agree with MMqPCR (correlation coefficent = 0.97, P < 0.001) [Ref].
______Repeat Diagnostics ($400.00) [Site, Links] info@repeatdiagnostics.com,
_________Original Procedure ($400.00): Telomere lengths are measured for lymphocytes and granulocytes via multicolor flow-FISH.
_________Detailed Procedure ($800.00): Telomere lengths are measured for lymphocytes and granulocytes PLUS B-cells, naïve and memory T-cells and NK cells.
_________Discounts are available for research contracts.
______Telome Health (Serves researchers, clinicians. Telephone for rates.)
______Life Length | Dr. Maria A. Blasco | Elsa Vera and Maria A. Blasco (2012), Beyond Average: potential for measurement of short telomeres, [PDF] Aging, V.4, No.6, June 6, 2012.
______
__Telomere Length Assay Services via
______RevGenetics Orders@RevGenetics.com,
______Terraternal info@terraternal.com,
______TA Sciences (TA Sciences/contact-us).
______Life Length info@lifelength.com.
_________Recharge Biomedical (Contact), administers testing via Life Length.
__Baseline "populations showed a highly significant decline as a function of client age in both
__lymphocyte [Images] and granulocyte telomere length by FlowFISH analysis [Images], and
__the slopes of the decline (55 and 34bp/year; p=10-15 and 10-8, respectively
"). - CB Harley, et.al, 2010.

Left: August 5, 2010 - Patient 71: Median Telomere Length measured = 6.3 kbp, Normal Median Telomere Length = 5.8 kbp (50th percentile line).
Right: April 5, 2011 - Patient 72: 2nd measurement, Median Telomere Length measured = 5.9 kbp, Normal Median Telomere Length = 5.7 kbp.
Here the median telomere decay with age is approximately (8.5 - 7.0)x1000/(70 - 30) = 37.5 bp/year. Patient lost 533 bp/year.
Note that telomeres in human germ cells are maintained at 15 kbp length. Max mammalian telomere length is about 30 kb, shortening 50-200 bp every cell cycle.

Left: August 5, 2010 - Patient 71: Median Telomere Length Measured = 8.3 kbp, Normal Median Telomere Length = 7.7 kbp (50th percentile line).
Right: April 5, 2011 - Patient 72: 2nd measurement, Median Telomere Length measured = 7.3 kbp, Normal Median Telomere Length = 7.7 kbp.
Here the median telomere decay with age is approximately (9.7 - 9.0)x1000/(70 - 30) = 17.5 bp/year. Patient lost 1,333 bp/year.

Above: y-axis = Telomere Length (proportional to kilobase pairs),
x-axis = Patient age in years. From Correspondent A ((Male, Age=71), 1st:August 5, 2010) and (Age 72) 2nd:April 5, 2011.
The spot size shows the error uncertainties. Note that the lines are cubic polynomial spline segments with 2 endpoints and 2 endpoint tangent vectors.
Shown as prepared by Terraternal, using the Repeat Diagnostics format also used by RevGenetics.

Colored lines refer to telomere measurements on a population sample.
In this case, the elderly patient (Correspondent A) used astragalus-derived telomerase activators [List], but telomere measures got worse.
A pre-existing kidney condition driving stress-induced senescence probably produced telomere attrition, as kidney disease typically does.
Correspondent A believed he was suffering from Radiation Nephritis lowering his glomerular filtration rate to about 30.
In the summer of 2012 we looked for a viral infection driving the fast telomere loss and found an HSV-1 (Herpes) infection.

Our results here seem to be consistent with VIDA Institute results on astragalus formulations.
When astragaloside IV was used for 6 months and average telomere lengths measured, VIDA Institute found
____Lymphocytes: 7.5-7.0 = reduction of 0.5 kb
____Granulocytes: 9.1-7.4 = reduction of 1.7 kb.
When cycloastragenol was used for 6 months, VIDA Institute found
____Lymphocytes: 7.0-7.0 = No change
____Granulocytes: 7.4-7.3 = reduction of 0.1 kb.
When Correspondent A used astragaloside IV and (for part of the 6 months) cycloastragenol, he measured
____Lymphocytes 6.3 - 5.9 = reduction of 0.4 kb.
____Granulocytes 8.3 - 7.3 = reduction of 1.0 kb.
See also the further discussion of VIDA Institute results by comparison.

Telomere Length Measurements in Leukocytes via Multi-Colored Flow-Fish
____[Links/Telomere Length Measurements in Leukocytes, Images, Papers, Patents, Books; Links/via multicolor flow-FISH, Images, Papers, Patents, Books].
____Baerlocher GM, Lansdorp PM (2003), Telomere length measurements in leukocyte subsets by automated multicolor flow-FISH, Cytometry A 55: 1-6, 2003.
____Telomere Length Analysis with Multi-Color Flow-FISH [Links, Images, Papers, Patents, Books, Amazon, at Repeat Diagnostics, Wiki/Flow FISH].

"Average Telomere Length by qPCR [Links, Images, Papers, Patents, Books] (quantitative polymerase chain reaction)
is by far the simplest and most scaleable assay for clinical Telomere Length measurements [Images, Papers, Patents, Books].
" - Telome Health, 2012.

New! Masayuki Kimura and Abraham Aviv (2011),
Measurement of Telomere DNA content by dot blot analysis,
Nucleic Acids Research, April 27, 2011, 1-7.
___"The qPCR [Links, Images, Papers, Patents, Books] and to a lesser extent
___Southern blots of TRFs (terminal restriction fragments) [Links, Images, Papers, Patents, Books]
___have been the main techniques used" in clinical research.

Single Telomere Length Analysis (STELA, PCR-based) [Links, Images, Papers, Patents, Books, Amazon].
Universal STELA [Links, Images, Papers, Books, Amazon].
___See Laila Bendix, Peer Bendix Horn, Uffe Birk Jensen, Ivica Rubelj and Steen Kolvraa (2010),
___The load of short telomeres, estimated by a new method, Universal STELA, correlates with number of senescent cells,
___Aging Cell (2010), 9, pp383-397.

Elsa Vera and Maria A. Blasco (2012),
Beyond average: potential for measurement of short telomeres,
Aging, June 2012, Vol.4, No.6., http://www.impactaging.com. (Overview, 2012).

Shay/Wright Laboratory Methods
Biowww.net/telomere and telomerase assays.
Therapy and diagnosis of conditions related to telomere length and/or telomerase activity by Michael D. West, WE Wright, and J.Shay, with useful refs.
Assessment of Telomere Length [Links, Images, Papers, Books] and factors that contribute to telomere stability [Links, Images, Papers, Books].
Telomere Length Measurements using Fluorescence Microscopy (PhD thesis) [Links, Images, Video, Papers, Patents, Books].
Telomere Length Measurements Using Digital Fluorescence Microscopy [Links, Images, Video, Papers, Patents, Books].
MMqPCR, monochrome multiplex qPCR method of telomere length measurement [Links, Images, Video, Papers, Patents, Books].
____Richard Cawthon (2009), Telomere Length Measurement by a novel monochrome multiplex quantitative PCR method [OnLine],
____Nucleic Acids Research, 2009 Feb; 37(3):e21.
____Jingjing Jiao, Jing X. Kang, Rui Tan, Jingdong Wang, Yu Zhang (2012),
____Multiplex time-reducing quantitative polymerase chain reaction assay for determination of telomere length in blood and tissue DNA, [OnLine],
____Anal Bioanal Chem (2012) 403:157-166.
Telomere Length Analysis via Telomere-Centromere FISH [Links, Images, Papers, Amazon; Links/telomere-centromere FISH; Links/FISH probes, Images].
Quantitative Fluorescence In Situ Hybridization (Q-FISH) [Links, Images, Papers, Patents, Books, Amazon].
Telomere Analysis with Metasystems Telomere Analysis Software [Links/Telomere Analysis Software, Images, Papers, Books].
Lansdorp Lab: Telomere Measurement Software (TFL-Telo)
Quantitative FISH image analysis [Links, Images, Papers, Books] (try Google & Adir).
Telomere Length Measurement via FISH & Flow Cytometry [Links, Images, Papers, Books].
Karyotype & Spectral Karyotype with Fluorescent In Situ Hybridization (FISH) [Links, Images, Papers, Books; Links/FISH probes, Images].
Telomere Length Measurement by Fluorescent In Situ Hybridization & Flow Cytometry: Tips & Pitfalls
Telomere Length Measurement with Q-Fish | TeloQuant Telomere Length Measurement Kit [Links/Telomere Length Measurement Kits, Images, Papers, Books].
Telomere Length Measurement in Blood Granulocytes [Links, Books, Papers; TA Sciences, Greta Blackburn Letter on 460 bp/year using Patton Protocol].
PubMED Telomere Length Measurement Links [Links, Images].
TRAP - Telomeric Repeat Amplification Protocol (Links) [Links, Images, Papers, Books], TRAP kits [Links, Images, Papers, Books].
Richard M. Cawthon, Telomere length measurement by a novel monochrome multiplex quantitative PCR method, Nucleic Acids Res 2009 February; 37(3).
See Biotechniques.com/A quantitative real-time PCR method for absolute telomere length for a Cawthon-method implementation.
See also Marcel E. Gil and Thérèsa L. Coetzer, (2004) Real-time quantitative PCR of telomere length, Molecular Biotechnology, Volume 27, Number 2, June, 2004 for another Cawthon implementation using a Roche LightCycler.
TeloTAGGG Telomere Length Assay (Roche) [Links, Images, Papers, Books].
HT-qFISH [Images, Papers, Books] based on automated confocal microscopy, can be obtained at this time through CNIO, in Madrid, Spain, for
__internuclear telomere length distributions [Images, Papers] and intranuclear telomere length distributions [Images, Papers]. For application, see
__Calvin B. Harley, Weimin Liu, Maria Blasco, Elsa Vera, William H. Andrews, Laura A. Briggs, and Joseph M. Raffaele (2010),
__A Natural Product Telomerase Activator As Part of a Health Maintenance Program, Rejuvenation Research, September 7, 2010.
_____Telomere length by FlowFISH [Links, Images, Papers, Books].
_____Telomere length by qPCR [Links, Images, Papers, Books].
_____Telomere length by automated high-throughput confocal microscopy (HT qFISH) [Links, Images, Papers, Books].

Immununohistochemical Staining [Books, Wikipedia].
Invitrogen Molecular Probes [Books] | Invitrogen Handbook | NIH: Innovation in Molecular Probes
Fluorescent Probes [Books].

Telomere Shortening and Senescence in Rat Brains [Books], telomeres and rat brains
Fluorescence Microscopes [Books] | Digital Cameras and Image-Processing Software for Microscopy [Books]
Robbins Scientific | Laboratory Automation Companies [Books]
Electron Microscopy in DNA sequence measurements [Books].

Six methods for measuring the length of telomere repeats are familiar:
(1) Telomere measurement by quantitative PCR [Books]. See also Telomere length measured at nucleotide resolution by gene sequencing after telomere PCR [Links, Papers]. See Marcel E. Gil and Theresa L. Coetzer, 2004: Real-time quantitative PCR of telomere length, Molecular Biotechnology, Humana Press Inc. Issue Vol 27, No. 2, June, 2004. See also Richard M. Cawthon, 2002: Telomere measurement by quantitative PCR, Nucleic Acids Research, 2002, Vol. 30, No. 10 e47, Oxford University Press.
Nanopore DNA Sequencing [Links] may be used on telomeres in the future. See Next_Big_Future/Nanopore Sequencing.
(2) Southern blot analysis of telomere restriction fragments. [Books, Papers].
(3) Quantitative fluorescence in situ hybridization with digital fluorescence microscopy (Q-FISH). See Chromosome Preparation for telomere FISH. (About 200 bp resolution.) [Links, Images, Books].
(4) Quantitative fluorescence in situ hybridization with flow cytometry (flow-FISH).
____[Books, Papers]
(5) Quantitative fluorescent in situ hybridization laser scanning cytometry (Q-FISHLSC) using a fluorescent-labeled peptide nucleic acid probe. For laser scanning cytometry for measuring fluorescent signals from LSC, see CompuCyte, Cambridge, Mass. [Books/Q-FISHLSC, Papers]
(6) Poll opinionated hamburger stand waitresses concerning your age. (A low-budget special), or use a mirror and ask yourself.
Use lines like ("Now how old do I look today, missy?") These ages are to be compared to "model ages"
computed in Age Transformation, and can be used to revise theoretical age transformation rates.
Then compute the theoretical telomere length rate of change from the observed age transformation rates.
See [Books/telomere measurement, Books/telomere length assessment, NCBI Papers].
[Books/Telomeres and Telomerase: Methods and Protocols, Links, Papers].
See Links/restriction endonucleases for telomere length research and Books/Endonucleases.
Links/restriction endonuclease sources. See New England Biolabs.
Books/human sub-telomeric sequences [Links].
Roche Telomere Length Assay Kit | Links/Telomere Length Kits | Links/Telomere Protocols
TeloQuant Telomere Length Kit
Repeat Diagnostics [See How Long are My Telomeres article from RevGenetics, mfg Astragaloside IV.] < $700 per measurement.

Telomerase Expression Measurements - TRAP - Telomerase Repeat Amplification Protocol (Links) [Books, Papers], TRAP kits [Links, Books, Papers]. TRAP is "the gold standard" in telomerase activation measurements, "but requires fresh or snap frozen samples for enzyme preservation. Alternative measures include RT-PCR for hRT and for hTERT mRNA." See also [Books/Telomerase Expression Measurements, Links, Links/Telomerase Measurements, Books/Telomerase Measurements].
TRAP assay for telomerase activation. [Links, Images, Video, Papers, Patents, Books].
[Books/Telomeres and Telomerase: Methods and Protocols, Links, Papers].
Allied Biotech, Inc. - Telomerase Detection Kits [Links, Books].
Telomerase enzyme activity [Books] requires expression of two genes [Links/GenBank],
hTRT [Books], the protein catalytic component gene, and
hTR [Books], the telomerase RNA component. [Books/measuring telomerase enzyme activity, Books/measuring telomerase activation, Links/telomerase-associated gene sequences].
See Telomerase-associated gene expression measurements via Northern Blot analysis
____[Links, Papers, (Links/Northern Blot analysis, Books)].
Telomerase-associated gene expression measurements via nuclease protection assays
____[Links, Papers, (Links/nuclease protection assays, Books, Papers)].
Telomerase component gene expression measurement via RT-PCR ISH
____[Links, Papers, (Links/RT-PCR ISH, Books, Papers)]
Quantitative Telomerase Detection Kit, US Biomax, Inc.
TeloTAGGG Telomerase PCR ELISA [Links, Images, Papers, Books; Links/ELISA].
(See also TeloTAGGG Telomere Length Assay (Roche) [Links, Images, Papers, Books].)
MILLIPORE TRAPEZE telomerase detection kit.
Links/Telomerase Kits | Links/Telomerase Protocols
See John P. Jakupciak, Peter E. Barker, Wendy Wang, Sudhir Srivastava and Donald H. Atha (2005),
___Preparation and Characterization of Candidate Reference Materials for Telomerase Assays,
___Clinical Chemistry 51: 1443-1450, 2005.

Telomere Articles & Links
The end sections of chromosomes (first described in 1888 by Waldeyer) were christened "telomeres" in 1938 by Herman Muller.
Hayflick and Moorehead published The Serial Cultivation of human diploid cell strains in 1961. [Review.]
The discovery of the telomere in Tetrahymena: Blackburn EH, Gall JG (1978).
A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. J Mol Biol. 1978 Mar 25;120(1):33-53.
Telomerase was first discovered in the ciliate Tetrahymena in 1985 by Greider and Blackburn [Papers/Carol Greider, Papers/Elizabeth Blackburn]. See Greider [Papers].
The TTAGGG or "T-TAG-GG" sequence in human chromosomes was first described in 1988 [Papers].
Reversible Cellular Senescence: implications for immortalization of normal human diploid fibroblasts. was first published by Woodring E. Wright and Jerry W. Shay in 1989.
CB Harley, Futcher, and Greider published Telomeres shorten during aging of human fibroblasts in 1990 in Nature 345: 458-460.
Then Extension of life-span by introduction of telomerase into normal human cells was published in 1998. [Links, Papers, Books].
Griffith, Comeau, and Rosenfield published Mammalian Telomeres end in a Large Duplex Loop in 1999, in Cell 97: 503-514.
Calvin B. Harley, et. al., Compositions and Methods for Increasing Telomerase Activity, 2005. (Geron patent ap.) [Links, Papers].
TelDB: Telomere Resources The NIH telomere information center. [Books, Links/telomeres].
Wikipedia on Telomeres | Telomere | Infoaging: Telomeres
Telomeres, Telomerase, and Cancer (A History)
Mapping and Initial Analysis of Human Subtelomeric Sequence Assemblies [Books]
t-loops at trypanosome telomeres [Links, Papers, Books] | t-loops at trypanosome telomeres (2)
T-loop assembly in vitro involves binding of TRF2 near the 3' telomeric overhang [Books].
Telomeres Do D-Loop T-Loop Minireview. New insights: Lariat structure in telomeres [Links, Images, Papers, Books].
Bidirectional replication from an internal ori site of the linear N15 plasmid prophage
Synthetic Telomeres & Electron Microscopy of Euplotes Telomerase [Books/Synthetic Telomeres]
How the balance between cells living longer and telomere health is kept without promoting the occurrence of malignancies.
The Mechanism of Telomere Synthesis by Telomerase [Links, Images, Papers Books]
Developmentally regulated initiation of DNA synthesis by telomerase: evidence for factor-assisted de novo telomere formation with links to other articles.
Telomere Synthesis | Lengthening Telomeres without Telomerase [Books]
Telomerase [Index, Books] | Telomerase at Davidson

Cancer and the Gene
See Index/Cancer, Index/Carcinogens, Index/Oncology, Index/Gene, Proto-oncogenes, Oncogenes, Index/Anticancer Drugs & Nutraceuticals.
Telomerase Inhibitors, Stabilizing G-quadruplex DNA - A New Cancer Cure? [Links, Images, Papers, Books] | Telomeres, Telomerase, and Cancer (A History) [Books].

Measuring Cellular Senescence
Cellular Senescence and Senescence Biomarkers from Senescence.info.
See Telomere Measurements, Index/Biomarkers of Aging, Index/Biomarkers of Longevity, Index/Replicative Senescence, Index/Cellular Senescence.
Testing for Cell Senescence [Books] | Cell Senescence Assay Kits [Books] | Cell Biolabs, Inc. | Cell Biolabs/Senescence
Links/Cell Senescence Kits [Links/detecting senescent cells, Images, Papers, Books] [Links/Cell Senescence Protocols, Images, Papers, Books].
Senescent Cell Morphology [Links, Images, Papers, Books; Hayflick, His Limit, and Cellular Aging, Images/young & senescent cells, Images/young & old].
___The Cytoplasm/Nucleus Size Ratio is larger for senescent cells [Images].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Telomere Lengths LifexLabs/Telomere Measurements.
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Beta-Galactosidase Detecting senescent cells with a stain sensitive to beta-galactosidase
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Lysosomal Beta-Galactosidase Senescence-associated β-galactosidase is lysosomal β-galactosidase Bo Yun, Jung A. Han, et al., Ageing Cell 5(2), 187 195.
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Heterochromatin foci formation [Links, Images, Papers, Patents, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
G1 phase arrest [Links, Images, Papers, Patents, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
p16INK4a [Index] is an accumulating senescence marker measurable with flow cytometry. [Links, Images, Video, Papers, Books, Article].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
p19ARF is upregulated in various tissues during mammalian aging. ARF regulates p53 stability. [Links, Images, Video, Papers, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
p21Waf1/Cip1 is an accumulating senescence marker.
___[Links/p21Waf1/Cip1 as a senescence marker, Images, Papers, Books; Links/p21/Waf1/Cip1, Images, Papers, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
CD28 [Index] is a senescence marker associated with T-cell senescence. [Links, Images, Video, Papers, Books; Links/CD28 as a senescence marker].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
SAG (senescence-associated gene) [Links, Images, Papers, Books]. SAG is a biomarker of cellular senescence.
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Phosphatidylcholine (Phosphatidylcholine = Lecithin), a cell membrane component [Images], is depleted in aging specimens [Images], dropping from
___a high of 90% in infancy to 10% in very elderly specimens. See Phosphatidylcholine measurements [Images], phosphatidylcholine protocols [Images].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Lipofuscin [Index, Wikipedia, Links, Images, Video, Papers, Books; Links/Lipofuscin Protocols; Links/Measuring Lipofuscin, Images, Papers, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Cell membrane lateral diffusion constant D in aging cells [Links, Images, Papers, Books, (The Membrane Hypothesis of Aging), (13)].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Membrane permeability to potassium in aging cells (nerve, liver cells) [Links, Images, Papers, Books, (The Membrane Hypothesis of Aging), (13)].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Membrane permeability for chlorine in aging cells (muscle cells) [Links, Images, Papers, Books, (The Membrane Hypothesis of Aging), (13)].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Cellular dehydration in senescent cells [Links, Images, Papers, Books, (The Membrane Hypothesis of Aging), (13)].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Mitochondrial Membrane Potential [Index, Links, Images, Papers, Patents, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
ROS [Index, Links, Images, Papers, Patents, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
___ROS levels are higher in senescent fibroblasts.
Actin structure patterns [Links, Images, Papers, Patents, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
___Actin patterns are different in senescent cells.
Senescent cells resist apoptotic signals [Links, Images, Papers, Patents, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Senescent cells resist EGF growth factor stimulation [Links, Images, Papers, Patents, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Senescent cells have a high level of DNA breaks [Links, Images, Papers, Patents, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Senescent cells have a high caveolin-1 levels [Links, Images, Papers, Patents, Books].
___[Protocols (Papers, Patents, Books), Assays, Kits, Stains, Software].
Telomere-dysfunction induced foci [Links, Images, Papers, Patents, Books].
____measured from gamma-H2AX accumulation on uncapped telomeres
___[Links, Images, Papers, Patents, Books].
GAPDH mRNA expression (glyceraldehyde 3-phosphate dehydrogenase mRNA expression)
___[Links, Images, Papers, Patents, Books].

Measuring Free Radicals, Free Radical Damage, and Oxidative Stress
Oxidative Stress [Index, Links/Testing for Oxidative Stress, Papers, Books].
Free Radical Theory of Aging [Index, Links/Testing for Free Radicals, Papers, Books] | Measuring free radicals in vivo [Links, Images Papers, Books].
Wiley: Measuring in vivo Oxidative Damage: A Practical Approach [Links, Images, Papers, Books].
Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?.
[Links/measuring oxidative damage in vivo, Images, Papers, Books]. | [Links/measuring oxidative damage in vitro, Images, Papers, Books].
Malondialdehyde Assay for Lipid Peroxidation | Cell Biolabs Oxidative Stress Test Assays
AMS Biotechnology Oxidative Stress Assays | Links/Oxidative Stress Kits | Links/Oxidative Stress Protocols
F2-isoprostane test for oxidative burden, $10.00 - Uses urine dipsticks you purchase and read at home. The F2-isoprostanes are prostaglandin-like compounds that are specific stable products of fatty acid oxidation (formed from oxidized arachidonic acid, a major membrane fatty acid) that are reduced by treatment with antioxidants.

Measuring DNA Repair Capacity [Links, Images, Papers, Books, LifeExtension].
Serum thiol test [Links, Images, Papers, Books]. Serum Thiol Test for DNA repair capacity, $65.00 - Draw blood or prick finger and send the test kit to a commercial lab for analysis. When oxidative stress is high, thiol bonds located within DNA folds of DNA repair enzymes are degraded by oxidation, so that the enzyme cannot repair your genes. "The thiol status of serum proteins and DNA repair enzymes has been associated with longevity in many species of mammals." - from The Anti-Aging Solution by Giampapa, Pero, and Zimmerman. In one study, treatment with CAEs (from Cat's Claw extract) was shown to reverse aging, as indicated by an increase in serum thiols.
AMS Biotechnology DNA Repair & DNA Damage Assays | Links/DNA Repair Assays
Links/DNA Repair Kits | Google/DNA Repair Protocols

Measuring Glycation
Links/Testing Glycation [Papers, Books, LifeExtension].
Links/glycation kits | Books/cellular glycation protocols.

Measuring Hormone Levels [Books, Links, Papers, LifeExtension].
Hormone Measurements from saliva for hormonal assessment of endocrine status, $25.00 per panel, test kit costs $10.00 (refundable with submission of a test panel), $30.00 for a single test. Collected at home and sent to a commercial lab. "Analysis determines the free and active hormones that reflect the free and unbound fraction in serum." One selects two panels. There are 2 for men and 2 for women, an anti-aging panel, and a competitive athlete panel. The anti-aging panel covers testosterone, cortisol, IgA, DHEA-S, Aldosterone, Thyroid T3, Thyroid T4, and melatonin.
Links/Hormone Kits | Books/Hormone Protocols

Measuring Lipofuscin
[Index/Lipofuscin; Links/detecting lipofuscin, Books; Links/measuring lipofuscin, Links/detecting ceroid, Books; Links/measuring ceroid, Books].

Clinical Testing | [Links/Clinical Testing Directories] Biocompare || Biowww.net || Clinician InfoRetriever || IndexB/Bioinformatics | IndexPR/Protocols
BioMarkers of Aging by Jennifer Krup, M.D. [Links, Images, Video, Papers, Books, Index/Biomarkers of Aging, Index/Biomarkers of Longevity].
Biomarkers of Aging, Ben Best. | Inner Age Biomarker Testing
Biomarkers of Longevity at Longevity Institute International. [Links, Images, Video, Papers, Books, Index/Biomarkers of Longevity, Index/Biomarkers of Aging].
Blood Screening [Index/Blood Tests, Links, Images, Papers, Books].
Blood Testing by the Life Extension Foundation [Index/Blood Tests, Books/Blood Testing, Books/Blood Testing in Anti-Aging Medicine].
Blood Granulocytes: Telomere Length Measurement [Links, Books, Papers; TA Sciences, Greta Blackburn Letter on 460 bp/year using Patton Protocol].
Clinical Chemistry (Books), Applied Clinical Chemistry (Books), Index/Medicinal Biochemistry, Index/Instrumental Analysis.
Clinical Testing [Books/Clinical Testing, Index/Blood Tests].
CD-28 measurements for T-lymphocyte senescence [Links, Images, Video, Papers, Books].
C-reactive protein testing, inflammation marker [Index/C-reactive Protein, Books; Index/Blood Tests].
Diagnosis Links [Links/Medical Diagnosis, Images, Books; Index/Blood Tests, Index/Cardiology, Index/Atherosclerosis, Index/Heart Attack, Index/Stroke].
DNA Sequencing [Index/SNPs].
FISH Telomere Measurement Technique on a Metaphase Spread [LifexLabs/Telomere Measurements, Images, Papers, Books].
Fluorescent In Situ Hybridization (FISH) [Wikipedia, Books, Amazon; Links/FISH probes, Images; IndexG/Gene, IndexB/Bioinformatics].
Fluorescent In Situ Hybridization technique for measurement of telomere length [Telomeasures, Images, Books, Amazon; Wikipedia/Telomeres, Images].
General Analytical Services Laboratories
Hematology [Index/Blood Tests, Wikipedia, Links, Books, Amazon].
Histology [Index/Histology, Wikipedia, Links, Books, Amazon].
Homocysteine Testing [Index/Homocysteine, Index/Blood Tests, Books] measures methylation effectiveness, toxic homocysteine levels.
Hormone Test Kit [Index/Blood Tests, Links, Books; Index/HGH, Index/IGF-1, Index/Estradiol, Index/Progesterone, Index/Testosterone]
Lipofuscin [Links/detecting lipofuscin, Books; Links/measuring lipofuscin, Links/detecting ceroid, Books; Links/measuring ceroid, Books].
Med Lab Tests online
Medical and Laboratory Sites | Lab Technologist.com - Breaking News on Lab Equipment [Books/Medical Laboratory Equipment].
MedicineNet Procedures and Tests
Metaphase Spread [Index, Images, Books, Amazon; Wikipedia/Cytogenetics; Links/Metaphase Spread Kits, Images].
Molecular Diagnostics [Links, Images, Papers, Books, Amazon].
Nutrition Bioanalysis Tests Testing Kits for Hair Mineral, Cholesterol, Blood Lipids, and Antioxidants. [Nutritional Ingredients, Analytical Lab [Books].
Pathology [Wikipedia, Links, Books, Amazon]
Private MD Lab Testing Online [Links/medical tests, Books/medical tests, Links/medical diagnostics].
Protocols [Index].
Roche - Cell Biology, Molecular Biology, Gene Expression, Disease & Drug
SA Biosciences [Links/Pathway Analysis, Books; Links/Gene Chips, Links/Transcripomics, Links/Systems biology].
SNPs [Index/DNA Sequencing].

Academic Laboratories & Programs [Google, Labome, Links/Academic Laboratories for Research on Aging, Images; ARC - Aging Research Center],
JenAge/Ageing-related Research Centres and Institutes, JenAge/Ageing-Related Organizations

American Aging Association
AFAR - American Federation for Aging Research
Aarhus University, Denmark, Suresh Rattan See the series The Biology of Aging and Its Modulation.
Albert Einstein College of Medicine - Longevity Genes Project
American Federation for Aging Research [Home, InfoAging].
The Artandi Lab at Stanford University. [Article].
Austad Lab: Dr Stephen N. Austad.
Barshop Institute for Longevity and Aging Studies at the UT Health Science Center, San Antonio, Texas.
The Huffington Center on Aging at the Baylor College of Medicine.
Blackburn Laboratory at the University of California at San Francisco. 2006 Lasker Foundation Award to Elizabeth Blackburn & Carol Greider for telomerase.
Brandeis University Lifespan Lab Links
The Buck Institute for Age Research [at http://www.buckinstitute.org/].
Campisi Lab, Ernst Orlando Lawrence Berkeley National Laboratory [Judith Campisi].
The Cech Lab of the Howard Hughes Medical Institute of the Univ. of Colorado at Boulder.
Danish Center for Molecular Gerontology [Books/Molecular Gerontology, Dr. Suresh Rattan].
de Lange Lab, [de Lange, Titia], Rockefeller University.
Ellison Medical Foundation
Harvard Molecular Technology and Computational Genetics Labs [Books/Molecular Technology, Books/Computational Genetics]
...Molecular and Cellular Biology at Harvard || Headline: New Harvard Medical School Anti-Aging Medicine Research Center.
Henry Wellcome Laboratory for Biogerontology Research, Newcastle on the Tyne, UK. [Books/Biogerontology]
Huffington Center on Aging, Baylor College of Medicine.
Institute for Aging and Health, Newcastle University, United Kingdom.
Institute for Biomedical Aging Research (IBA), Austrian Academy of Sciences.
Institute of Ageing and Chronic Disease at the University of Liverpool.
Institute of Gerontology at Wayne State University in Detroit, Michigan.
Institute of Healthy Ageing at University College London.
Institute of Molecular Gerontology/NIH/GOV
International Center for Genetic Engineering and Biotechnology [Home].
Jackson Laboratory: The Aging Center.
Karlseder Lab - Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies.
Laboratory of Experimental Gerontology - investigations, American Aging Association
Leibniz Institute for Age Research (at the Fritz Lipmann Institute).
Marine Biological Laboratories/Course: Molecular Biology of Aging with Caleb E. Finch.
Max Planck Institute for Demographic Research.
Max Planck Institute for Biology of Ageing.
Molecular Gerontology, Schools of
Mount Sinai Neurobiology of Aging Laboratories [Links].
National Institute on Aging NIH/NIA at http://www.nia.nih.gov/.
Oak Ridge National Laboratory - Telomere Quantification by Florescence In Situ Hybridization - Software.
SENS from Aubrey de Grey.
Shay/Wright Laboratory at the University of Texas Medical Center [map] - Optical Imaging Core [Woodring E. Wright, Jerry W. Shay].
Simon Fraser University, Canada Gerontology Progams and Research Centers in Canada.
St. Petersburg Institute of Bioregulation and Gerontology [Eng.Home, map] [Home Page, About Us, Links/Schools of Bioregulation and Gerontology].
Szostak Labs - Jack W. Szostak - Lasker Award with Greider and Blackburn. [Article with photo].
Titia de Lange Lab [de Lange, Titia], Rockefeller University.
UCLA Molecular Genetics Links.
University of Aarhus, Denmark, Suresh Rattan See the series The Biology of Aging and Its Modulation.
University of Arkansas - Deptartment of Geriatrics.
University of Chicago: Clinical Services - Telomere Analysis, FISH probes.
University of Groningen (Netherlands) [maps].
University of Idaho - Austad Lab,.
University of Michigan Institute of Gerontology.
University of North Carolina at Chapel Hill, Department of Biochemistry and Biophysics.
USC Davis School of Gerontology, Caleb E. Finch [The Biology of Human Longevity].
Tokyo Metropolitan Institute of Gerontology.
University of Michigan Geriatrics Center & Institute of Gerontology. See also the Institute of Gerontology.
University of Washington - Basic Biology of Aging, Healthy Aging Research Network.
Univeristy of Wisconsin - Judd Aiken - Role of mitochondria in aging.
US Schools of Molecular Biology [Links].
Wayne State University - Institute of Gerontology - Robert Arking [Biology of Aging: Observations and Principles].
West Virginia University Center on Aging || Penn State Gerontology Links.
WISTAR Institute [home]: Novel Telomerase Activators [Links] have been proposed in Israel at the WISTAR Institute.
____based on the design of molecules to regulate telomerase. See WISTAR professor
____Emmanuel Skordalakes PhD [Links, Papers, Images].
Who's Who in Gerontology
___Judd Aiken, Bruce Ames [Links, University of California],
___Robert Arking [Biology of Aging: Observations and Principles], Steven N. Austad [Amazon],
___Gustavo Barja [Books, Amazon], Andrzej Bartke [Message, Links], Dr. Nir Barzilai, M.D. [Beeson Institute/Dr. Nir Barzilai]
___Seymour Benzer [Caltech Lab, Books], Ben Best [Links],
___Elizabeth Blackburn [Blackburn Lab, Links, Books, Images, Amazon, Papers, Patents],
___Maria A. Blasco [Links, Papers], Vilhelm A. Bohr [National Institute on Aging, Links, Books]....

Symposia and Conferences in Anti-Aging Medicine [Links, Select Biosciences] JenAge/Meetings.
The Tenth International Symposium on Neurobiology and Neuroendocrinology of Aging, July 25–30, 2010, Bregenz, Austria.

Vendors | GEN | Biocompare | Biotech-Web | Daigger | LabEquip | Links/biotech lab equip | Links/medical lab equip | Biowww.net || Google | Yahoo
23andMe - Personal genetics or personal genomics with application to diseases.
4SC - 4SC develops innovative, orally administered small molecule drugs for autoimmune diseases and cancer.
454 Life Sciences - Rapid DNA sequencing [Wikipedia, Index, Links/DNA Sequencing, Images, Video Papers, Books].
Abbott Laboratories/VYSIS, Abbott Molecular Inc.
abcam - Antibodies, Proteins, Peptides, Elisa kits, Assay Kits, Immunostaining reagents, Scientific Support.
ABEC - Designs and manufactures production equipment for pilot and commercial scale biopharmaceutical manufacturing.
Achillion Pharmaceuticals - Biopharmaceutical solutions for infectious disease including HCV and resistant bacterial infections.
abm - ABM hTERT cell immortalization reagents
Active Motif - Tools to analyze and measure [Links, Books] functions in the cell nucleus [Books, Links].
addgene (plasmids), addgene/hTERT, pBABE-neo-hTERT plasmid (52) (plasmid 1774), needs mods [Ref].
Advanced Cell Technology - Embryonic stem cell technology.
Agilent Technologies - Life Science Lab Products, electronics, and oligotide microarrays.
Allied Biotech, Inc. - Telomerase Detection Kits [Links, Images, Papers, Books]
Alnylam Pharmaceuticals - Specializes in RNA interference (RNAi) with RNAi technology for silencing disease-causing genes.
Altogen Polymer-, Lipid-, and Nanoparticle-based transfection technologies.
Ambion (Austin, TX, USA) The RNA company [Products, Links]; RNA polymerase, MEGAscript T7 Kit.
___ GeneAssist Pathway Atlas - The molecular biology of the cell: "signal transduction, metabolic and disease state pathway maps, as well as their descriptions and relevant references. When a protein is selected from one of the maps, a detailed view appears showing additional gene information along with the recommended Silencer siRNAs and corresponding TaqMan Gene Expression Assays for studying that particular gene."
Amersham Biosciences, GE Healthcare Products, [Amersham RNA labeling kit], [Amersham Pharmacia Biotech, Restriction Enzymes].
AMGEN - Protein therapeutics.
Amplicon Express - DNA sequencing [Books] and other bioservices, cloning and expression vectors, ect. [Books].
AMS Biotechnology - Research products for genomics, proteomics and immunology, including assay kits, cDNA, RNA, microarrays, western blots, polyclonal antibodies, monoclonal antibodies, antigens & ELISA kits.
Anadys - Develops novel medicines for the treatment of hepatitis C [Wikipedia].
Analytical Lab - Nutritional Ingredients
Antibodies-online.com [Links/Antibodies, Books, Amazon, Wikipedia/Antibody]
Antagene Custom Antibody & Peptide Synthesis - Peptide synthesis service.
Aphelion Imaging software toolkits useful in telomere FISH work.
Applied Biosystems - Gene Sequencing apparatus and other bioscience apparatus [Books] & Telomere Extension by Telomerase Pathway Atlas
Applied Imaging [Links] Cytovision.
Aquila BioMedical - Multiple Sclerosis (MS), inhibiting the autoimmune/inflammatory phase of MS and/or providing neuroprotection and remyelination.
- Specialists in neuroscience, myelination and remyelination, regenerative medicine, neuroimmunology and inflammation biology.
Arena Pharmaceuticals - Develops pharmaceuticals.
Array Biopharma - Discovers and develops small molecule drugs targeted for cancer and inflammatory diseases.
Artel - Pipette calibration - Liquid handling equipment and methods for superior assay results.
ATCC - American Type Culture Collection - Cell lines for testing, such as telomerase activation testing. Plasmid source.
ATCC Bioproducts - Cell Immortalization via hTERT plasmids - Immortalization of Cells in Culture. [Books] || Serotec Antibodies.
Atox Bio - A clinical stage biotechnology company that develops peptides and small molecules for infectious and inflammatory/autoimmune diseases.
Bayer
Beckman Coulter, Inc. - Diagnostics, Biomedical Research Applications.
BD - Becton, Dickinson, and Company [Products], manufacturer of Vacutainers for phlebotomy, syringes; mouse collagen for coatings; flow cytometry, ect.
BD Biosciences.
BigDNA
Bioanalytical Systems
Biocarta PATHWAYS: Telomeres, Telomerase, and Immortality.
Biogen Idec
Biogenova: Lentivirus Products - LG508 Lenti-hTERT-eGFP immortalizes primary cells.
Biolab equipment, advanced [Books], Polymerase Chain Reaction kits, ect. [Books].
Biologics Process Development
Biomerix
BIOMOL on-line catalog.
Bionex Solutions
Biopsy Equipment [Links/biopsy equipment, Links/fine needle aspiration biopsy equipment].
Bio-Rad Laboratories - Life Science Research, Clinical Diagnostics, Informatics, Separations, microfluidics (QuantaLife), image analyzers.
Biosource International
Biotechnology Links [Books], Biotechnology Equipment links [Books].
Biotique Systems
Biovest International
Bio-World.com.
Blue Heron Biotechnology - Synthesize genes and synthetic DNA sequences economically and with perfect accuracy with
Blue Heron Bio's GeneMaker proprietary, novel and high throughput design and synthesis platform.
Boehringer Ingelheim
Brinkman Instruments - Bio & Analytical Chemistry lab equipment.
Bristol-Myers Squibb - Medicines, medical imaging.
Bruker X-ray diffraction apparatus for x-ray diffraction determination of structure [Books/apparatus, Books/structure]
Caliper Life Sciences - Products by application - Drug Discovery, Drug Development, Genomics, Proteomics.
Cambridge Research Biochemicals
CRi - Cambridge Research & Instrumentation - "Oosight" system for nuclear transfer cloning, recently used to clone monkeys.
CamScan Scanning Electron Microscopes [Books]
Cambrex - Pharmaceutical and Bioscience Products - About Cambrex
Carolina Biological Supply Company Materials to support DNA Science, Dolan DNA Learning Center of the Cold Spring Harbor Laboratory.
Cascade Biologics - Human cell cultures, for telomerase activation testing, for instance.
__"Telomerase activity was measured in human neonatal keratinocytes (Cascade Biologics, Portland, OR)
__and in MRC5 fetal human fibroblasts (ATCC# CCL-171) pre-and posttreatment in culture
__with TA-65® using the telomere repeat amplification protocol (TRAP) assay..
" - CB Harley, et.al, 2010.
Cell Biolabs - Oxidative Stess Assays, similar Assays for Science Research
Cell Culturing Equipment (Links) [Books/Cell Culturing, Amazon/cell culturing]
Cell Culturing Technologies [Books].
Cellecta
Cellectis bioresearch
Cellon
CELLSCRIPTTM Kits for supporting the preparation of nucleoside-modified therapeutic mRNA. See Fast Telomere Extension.
Cell Signaling Technologies (Danvers, MA, USA) Monoclonal antiphosphopaxillin.
___Protein may be harvested by washing cells once with PBS and then by lysing cells in RIPA buffer.
Cell-Sorting [Books, Amazon].
Cell-Sorting Machine Vendors [Links, Books, Papers] | Cell-Sorting & Mitochondrial Aging
Cellular Dynamics International (Site) Fabricates iPSC cells with episomal vectors which are then specialized to specific cell types for drug test applications.
Cempra Pharmaceuticals
Centrifuges [Books].
Cepheid
Chemical Laboratory Supplies Net
CerCell
Cerner
Chromatography [Books] | High Performance Liquid chromatography [Books] | Affinity Chromatography [Books]
Clinical Chemistry Equipment (Links)
Cliniplace Worldwide
Complete Genomics
Conatus
Creative-Animodel.com. Animal models including non-human primates.
Cryostats [Books], Cryosection Technique [Books], Cryosection Protocol
CrystalGenomics
Curetis
Cytheris
CytRx
Daiichi Pure Chemicals - Life Science Products.
DebioPharma Group
Dharmacon (gelifesciences/dharmacon): Custom RNA synthesis, Gene Expression (overexpress gene of interest), Technical Resources, app notes, gene pathways, protocols, tips, videos.
Provides siRNA duplexes, 21-nucleotide siRNA duplexes.
DNA 2.0 - Synthetic Genes, Gene Design Software. [Books/Gene Design, Links].
Dupont
Eisai
Elan
Epicentre Biotechnologies (Products)
EMD Biosciences: Calbiochem, Novabiochem, Novagen: Products for: Protein Phosphorylation/Dephosphorylation, Cell Cycle & Apoptosis,
___Drug Discovery, Proteomics, Calcium Signaling, Nitric Oxide & Oxidative Stress,
___Cell Adhesion & Extracellular Matrix, G-proteins, Proteases, Lipid Signaling, Neurobiology, Cytokines & Growth Factors.
EMD Millipore, Billerica, MA, USA: Trapeze Kit. The time course of telomerase activity can be determined using the TRAPeze RT kit.
Enzo Life Sciences
Enzon Pharmaceuticals
Eppendorf products: Cell Manipulation, Centrifugation, Lab Automation, Microarrays, PCR, ect.
European Collection of Cell Cultures - ECACC
Evotec
ExChemistry.com
Faster Cures - The Center for Accelerating Medical Solutions
FIOS Genomics
Fisher Scientific: Life Science, Food Diagnostics, Chemicals, Organics, Science Ed., Healthcare, ect.
Fluidigm: Array IFCs for real-time PCR, digital PCR, single-cell gene expression, next-generation sequencing sample preparation, SNP analysis and more.
Fluorescence Microscopes [Books] | Digital Cameras and Image-Processing Software for Microscopy [Books]
Garden State Nutritionals - Packages TA-65 into capsules for TA Sciences, tests new drugs.
GE Healthcare Products.
Gel Electrophoresis [Books], SDS Gel Electrophoresis [Books, History & Methods],
Gene Link long oligos, fluorescent molecular probes, ultra-modified DNA, RNA, Chimeric, Fluorescent, and Antisense Oligos.
Genentech - Leading provider of anti-tumor therapeutics in the United States, biotechnology.
Genscript - Gene, Peptide, Protein, and Antibody Services. Could synthesize Epithalon Peptide, for instance. Protein product pipeline available.
genzyme - Protein and antibody therapies, polymers and small molecule therapies, cell and gene therapies, and biomaterials.
Geron [Wikipedia, Links] - Telomere & telomerase technologies. [patents, European patent/telomerase activation methods, European patent/Astralagus extracts(for Hong Kong U.)].
Gilead
Gilson
Glassware, Scientific and Laboratory [Books] || Surplus Lab || Best Lab Deals
GlaxoSmithKline
Glenmark Generics
Global Scientific Supply - General Biolab and Chemlab equipment.
GlobeImmune
GMI - Lab Instrumentation (many brands)
Goodwin Biotechnology
Graftys
Greyfield Optical Inc. Online Videos, microscopy equipment.
Grizzly Analytical
Gyros
Hach Co..
Hamilton
Hammamatsu Photonics - Hammamatsu Ora Camera - used in Shay/Wright Telomere/Centromere FISH.
Henogen - Biomanufacturing and pharmaceuticals. Acquired 4C Biotech, a source of human cell cultures.
Hudson Robotics
HGS - Human Genome Sciences - Biopharmaceuticals, including drugs to treat hepatitis C, lupus, anthrax disease, cancer, rheumatoid arthritis and HIV/AIDS.
Idenex
IDT - Integrated DNA Technologies (catalog), Custom gene synthesis, IDT SciTools.
Illumina Gene Expression Product Portfolio - [Links/whole genome bead array technology, Books, Papers]
Imgenex [Products] - Innovations in functional genomics.
ImmunID
Immunohistochemical staining kits [Books]
Incyte
Indigo Biosciences The Nuclear Receptor Company. [Links/Nuclear Receptors, Links/Transcription Factors].
InPhotonics, Inc. - Raman spectroscopy.
Integrated DNA Technologies
InterMune
Invitrogen | Invitrogen Products | Focus Areas | Kinase Biology | Human Biological Pathways | Lipofectin for mRNA transfections.(PDF ref). See Fast Telomere Extension.
InvivoGen Plasmid source, cell lines.
Isis Pharmaceuticals
Jackson Immunology HRP-conjugated donkey anti-mouse Ig.
Jenway
Johnson & Johnson
Kaiser Optical Systems, Inc. - Raman Spectroscopy.
Laboratory Supply Company
Labtec
Leeman Labs (Teledyne) - Atomic spectroscopy.
LG Life Sciences
Lifescan, Inc. - Glucose monitoring.
Life Technologies (Grand Island, NY, USA): SYBR Gold Nucleic Acid Gel Stain.
___Cells may be transfected with nucleoside-modified hTERT mRNA using Lipofectamine RNAiMax
___prepared in Opti-MEM Reduced Serum Media and added to the cells in a 1:5 v:v ratio
___with their normal media to achieve final concentrations for Fast Telomere Extension [Ref].
___GM847 cells can be obtained from SACRIAntibody Services, University of Calgary [Ref].
LightCycler Systems for Real-time PCR from Roche Applied Science
Lilly - Eli Lilly & Co., biopharmaceuticals, R&D.
Lonza, Allendale, NJ, USA, Human 30-yr-old primary skeletal muscle myoblasts, to be cultured in SkGM-2 media (Lonza).
Lotion Crafter - Wholesale supplier of natural materials for the development of cosmetics and toiletries.
Marlingen Biosciences [Products] - Multiple transcription factor identification, mitochondrial DNA assays, ect.
MCLAB advanced bioservices and products- with free on-line software tools.
McKinley Scientific Laboratory Supplies [Books].
Medical Equipment Links & related | Medical Device Resource Center | Medical Equipment (Links).
Medical Laboratory Equipment (Links).
MedImmune
Meissner
Merck.
Merck Serono.
Mettler Toledo, Inc. Lab Equipment.
Microbiology Equipment (Links)
Microcentrifuges [Links, Images, Books].
MicroPhotonics X-Ray Diffrac.
Microtomes [Wikipedia, Books], Ultramicrotome [Links, Books].
MILLIPORE - Tools for bioscience research and biopharmaceutical manufacturing. TRAPEZE telomerase detection kit; Muse Cell Analyzer.
Mirus Bio The Transfection Experts. See TransIT mRNA for mRNA transfections (PDF ref). See Fast Telomere Extension.
Molecular Diagnostics [Images, Papers, Books, Amazon]: [Roche, Abbott, Gen-Probe, Qiagen, BD, Siemens, Cepheid],
___molecular markers for cancer, cardiac disease, diabetes, arthritis, neurological diseases, and psychiatric conditions.
Molecular Dynamics PhosphorImager, ImageQuaNT Workstation [Images]. "PhosphorImager SL [Images]".
Molecular Probes (Invitrogen). See also Links/Molecular Probes.
Mouse and Hamster Racks [Books/Mouse and Hamster Lab Procedures].
NanoBio
National Instruments.
New England Biolabs - Now to include Rubicon Genomics technology for amplification of DNA from single cells.
___New England Biolabs, Ipswitch, MA,USA: Nucleoside-modified materials for Fast Telomere Extension, Antarctic Phosphatase.
New England Peptide
NIH Image Processing software - used in FISH telomere studies. [Links, Books].
Nikkon Microscopy (Nikkon USA) - Microscopy U. - Microscopy Tutorials, Image Galleries, Fluorescence Microscopy, ect.
NIMGenetics
Novartis
Novasep Biologics from gene to drug product.
Novo Nordisk
Novus Biologicals
Nycomed
Ocean Optics - Optical sensing and spectroscopy.
Oligo Factory [Mfg oligonucleotides] - Manufactures DNA oligotides & RNA oligotides, dual-labeled probes, and exotic oligos.
Orflow Technologies
Origene: Genome-Wide cDNA clones & Protein production, Gene Synthesis, Genome-Wide shRNA, siRNA, and miRNA.
Orphazyme
Otsuka Pharmaceutical
Oxigene
Pacific Biosciences
Pacira Pharmaceuticals
Pall Corporation - Laboratory Products - Filtration, Separation & Purification, Life Sciences Products.
PCR amplification kits [Books, Links, Fast PCR Tutorial]
PDL BioPharma - IV Busulfex to support allogeneic hematopoietic stem cell transplantation, biopharamaceuticals.
Peptide Synthesis, Custom [Books, Papers], Peptide Synthesis [Wikipedia, Books], Peptide Sythesizers [Books, Suppliers]
Perkin-Elmer Life and Analytical Sciences - High Throughput Screening [Links, Wikipedia, Books, Amazon].
Pfenex
Pfizer - Pharmaceuticals and pharmaceutical research.
Pharmacological Lab Equipment Suppliers [Books]
Pharmasset
Pharmigene
Phoenix Biomolecular - Telomerase activation therapies.
Pierce Chemiluminescent detection systems.
Postnova Analytics - Advanced analytical products.
Prevention Trials Network
Promega Automated analysis systems, Cellular Analysis, DNA & RNA analysis, Drug Discovery, Genetic Identity, Molecular Diagnostics.
___firefly luciferase and Renilla luciferase enzyme activity assay systems.
PromoCell The Cell Culture Experts.
PromoCell Academy Training Courses.
PromoKine.info Cell Research Partner.
Proquinase Tools and Testing.
ProSpec Protein Specialists - Provide some exotic telomerase activators, such as Interleukin-2, EGF, and PDGF.
Protein Technologies
Prozyme
PsychoGenics
QIAGEN Sample and assay technologies for biomedical and drug development, ect, [Links/Molecular Diagnostics].
___RNA isolation using RNeasy (Qiagen).
QuantaLife: Droplet digital PCR genetic analysis.
R & D Systems
Rafagen
Rainin Instruments Pipetting Solutions
Raumedic
Reinervate - 3D cell culture technology based on alvetex, a polystyrene material for cell culturing.
Repeat Diagnostics [See How Long are My Telomeres article from RevGenetics, mfg Astragaloside IV.]
Robbins Scientific | Laboratory Automation Companies [Books]
Roche - Cell Biology, Molecular Biology, Gene Expression, Disease & Drug Research
- [Books/Cell Biology, Books/Molecular Biology, Books/Gene Expression, Books/Disease Research, Books/Drug Research].
Roche Applied Science Protease inhibitor.
Roslin Cells
Rules-Based Medicine
SA Biosciences [Links/Pathway Analysis, Books; Links/Gene Chips, Links/Transcripomics, Links/Systems biology].
Samsung Biologics (Site): Biopharmaceutical Manufacturing - Contract manufacturing plus Samsung biopharmaceutical products.
Sanofi
Santa Cruz Biotechnology Offers bpV(pic), and many other bioscience products, monoclonal antibodies against phospho-FAK, Cdc42, and actin.
Santaris Pharma
Sarstedt
Sartorius Stedim Biotech
Satoris
Scynexis
SDS Gel Electrophoresis [Books], Gel Electrophoresis [Books].
Serva - Electrophoresis Reagents, Equipment
Shire
Siemens Healthcare
Sidec - Protein tomography with electron microscopy.
Sierra Sciences [Wikipedia, Links] - Developing medicines and therapies that will reverse the effects of human aging; telomerase activation research.
Sigma Aldrich - Life Science, Analytical/Chromatography, Chemistry, Biochemical reagents, ect.
Sigma Life Science: CompoZr ZFN technology for Zinc Finger Nucleases applied to targeted genome editing.
Spectro Analytical Instruments
Staining Apparatus for Histology and Light Microscopy [Links, Images, Books/..for microscopy, Books/..for histology]
Stem Cell Culturing Equipment (Google Links)
Sterilization Equipment (Google Links)
Stratagene DNA Extraction Kit used in Telomere/Centomere FISH.
SuppreMol
Surgical Equipment (Google Links)
Swagelok
Syn Bio Research in synthetic biology: Designing bacteria to attack cancer cells.
Syngene
Syngenta
Synthecon 3D Cell Culture Systems [Books] | Cell Culturing Technologies [Books]
T. A. Instruments - Thermal Analysis Instruments.
Takeda Pharmaceutical
TA Sciences [Links] - Telomerase Activation Sciences. - TA-65, probably cycloastragenol, from astragalus extract [Forum].
Tecan
Tekmira Pharmaceuticals
Telomolecular Nanotechnologies (defunct) [Links]. Telomerase Activation Techniques and Life Extension Methods.
_____Telomolecular Nanotechnologies Corporate Video.
Thermo Scientific - Biolab equipment.
Titertek-Berthold
Trade Shows Eventseye: Trade Shows Worldwide, Links/Trade Shows USA, Biz Trade Shows, TS Event Resource, TS Scratchpad.
Transduction Laboratories (Lexington, KY). monoclonal antibodies against caveolin-1 and other proteins.
Trevigen - Bioscience Test Protocols for Cancer Research; DNA Damage, ect.
TriLink Biotechnologies provides nucleoside-modified mRNA, which is used in Fast Telomere Extension, iPS cell technology, ect.
___Provides custom mixes of canonical and noncanonical nucleotides.
TTP LabTech
UCB
US Biomax, Inc. - Quantitative Telomerase Detection Kits, plus Tissue Arrays, Tissue microarrays, Tissue Banks, and Protein microarrays.
Varian - Information Rich Detection, Chromatography, Spectroscopy, Vacuum Technologies.
Vector Laboratories - Immunohistochemical, biomedical labeling & detection systems.
Vertex
Viropro
Waco Pure Chemical Industries
WatchFrog
Waters
Watson Pharmaceuticals
Whatman Separation technologies.
Wyeth BioPharma - Development and manufacturing of recombinant protein biopharmaceuticals.
Zeiss Microscopy & Image Analysis [Links, Images, Video, Papers, Books, Yahoo Links] - Axioplan 2 Fluorescence Microscope, used by Shay & Wright.
Zymed Laboratories Secondary horseradish peroxidase-conjugated anti-rabbit and anti-mouse antibodies.

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Clinician InfoRetriever | The Integrated Taxonomic Information System includes medicinal plants such as astragalus.

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