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Highlight Links
Telomere Measurements
Telomerase Expression Measurements
Telomere Articles and Links
Cancer and the Gene
Measuring Cellular Senescence
Clinical Testing
Academic Laboratories and Programs
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Laboratory Equipment & Material Vendors
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Cell Culturing

Index Themes Analytical Chemistry | Bioinformatics | Biomarkers of Longevity and Aging | Human Genome | Instrumental Analysis | Protocols | Medical

Cell Culturing
___ See also Culture of Animal Cells by R. Ian Freshney, Wiley-Liss, 5th edition, 2005.
[Links, Images, Video, Papers, Books, Amazon, Wikipedia, Wiki/Cell Culture].
Note that the in vitro lifetime of cultured human cells [Images] is typically < 20 weeks. The cell cycle repeats so frequently that cells often die of replicative senescence after about 50 cell divisions in 14 weeks. This may not be enough time for slow-acting small molecule telomerase activators like astragaloside IV to make much difference at the usual concentrations used in treatment, although the positive effect on wound healing is visible. On the other hand, with suitable provisions for activating transcription of hTERT mRNA via telomerase-activating measures, the cells can become immortal. An extra copy of the hTERT gene transfected with a viral vector will immortalize many, if not all, human cell types. See Hayflick, His Limit, and Cellar Ageing by Jerry W. Shay and Woodring E. Wright.

Agar [Wikipedia/Agar, Links; Links/Agar plate, Images, Video, Papers, Patents, Books; Wikipedia/Agar_plate]
___Invitrogen/Gibco, Difco.
Amino Acids [Wikipedia, Links, Images, Video, Books] Sigma.
Aminopterin [Links, Images] Sigma.
Amphotericin B [Links, Images, Video, Papers, Books] BioWhittaker, ICN, Sigma.
Ampules, glass [Links, Images, Video, Papers, Books] Wheaton.
Ampules, plastic [Links, Images, Video]
___Nalge Nunc (Gibco in U.K., Fisher Scientific subsidiary in USA.), Corning Costar.
Anemometers [Links, Images, Wikipedia].
___See laminar-flow hoods [Links, Images, Video, Papers, Patents, Books].
Animal Cells [Wikipedia/Animal cells, Links, Images, Video, Papers, Books;
__Links/Animal Cell Cultures, Images, Video, Papers, Books;
__Links/Animal Stem Cells, Images, Video, Papers, Books;
__Links/Animal Stem Cell Cultures, Images, Video, Papers, Books;
__Links/Animal Cancer Cells, Images, Video, Papers, Books;
__Links/Animal Cancer Cell Cultures, Images, Video, Papers, Books;
__Links/Animal Senescent Cells, Images, Video, Papers, Books;
__Links/Animal Cell Protocols, Images, Video, Papers, Books].
Antibiotics [Index, Wikipedia, Links, Images, Video, Papers, Books, Amazon; Links/Over-The-Counter].
Antibodies [Wikipedia, Links, Images, Video, Papers, Books].
___Amersham, Biopool, Boehringer, DAKO, Invitrogen/Gibco, ICN, R&D, Serotec, Sigma,
___Upstate Biotechnology, Vector.
Antifoam RD emulsion 9964.40 Dow-Corning.
Antifoams [Links, Images, Video; Links/for the Cell Biolab, Images] Merck, Miles, Sigma.
Automatic Pipettes [Links, Images, Video]
___Alpha, Becton Dickinson, Boehringer, Corning Costar Gilson, ICN, Jencons, Labsystems, Rainin.
Automatic pipette plugger [Links, Images] Bellco, Volac (See Camlab).
Autoradiographic Emulsion [Links, Images, Video, Wikipedia/autoradiography]
___See emulsion for autoradiography.
Bactopeptone [Links, Images] Difco, Invitrogen/Gibco (Nalge Nunc).
Benchcote [Links, Images] Johnson & Johnson.
Biochemicals [Links, Images, Wikipedia]
___Boehringer, Merck, Calbiochem, Fluka, Pierce, Sigma, U.S. Biochemical.
Biopsy [Index, Wikipedia, Links, Books, Amazon; Links/biopsy instruments].
Bioreactors [Links/Bioreactors, Images, Papers, Books, Wikipedia; Links/Single Use Bioreactors, Images, Papers, Books; Index/Protein Production for Recombinant Proteins; Links/Bioreactors for Recombinant Protein Production, Images, Papers, Books]. See also fermentors [Links, Images, Wikipedia].
Bottles [Links, Images, Wikipedia]. Bellco, Corning, Fisher, Schott.
Bovine Serum Albumin [Links, Images, Wikipedia/Albumin].
___Amersham, Bayer, Biofluids, Becton Dickenson, Calbiochem, ICN,
___Invitrogen/Gibco (Nalge Nunc), Intergen, Sigma.
Camera lucida [Links, Images, Video, Wikipedia/Camera Lucida]. Leica, Olympus, Nikon, Zeiss.
Carboxymethyl-cellulose (CMC) [Links, Images, Wikipedia] Fisher, Merck.
CCD Cameras [Links, Images, Video, Wikipedia/Charge-coupled_device].
___Bioworld, Dage-MTI, Hamamatsu, Leica, Scanalytics.
Cell Banks [Links, Images, Video; Links/for the Cell Biolab, Images, Video]
___ATCC, Coriell, ECACC, DSMZ, JCRB, Riken.
Cell Counters [Links, Images, Video, Wikipedia] Beckman Coulter, BioWorld, Scharfe.
Cell scraper [Links, Images] Becton Dickenson, Corning Costar Nalge Nunc (Invitrogen/Gibco).
Cell sizing [Links, Images] Beckman Coulter, Sharfe.
Cell strainers [Links, Images] Beckton Dickenson, Dynal, Miltenyi, Biotec.
Centrifugal elutriator [Links, Images] Beckman.
Centrifuges [Links, Images, Video, Wikipedia/Centrifuge].
___Beckman, Fisher, Heraeus, IEC, Life Sciences International, Sorval (Dupont).
Centrifuge Tubes [Links, Images] Alpha, Corning, Falcon (Becton Dickenson), Nalge Nunc (Invitrogen/Gibco).
Chamber Slides [Links, Images] Applied Scientific, Bayer, Becton Dickenson, Bibby Sterilin,
___Heraeus, Metachem, Nalge Nunc (Invitrogen/Gibco), Stem Cell Technologies.
Chemicals [Links, Images, Wikipedia; Links, Images] Merck, Boehringer, Calbiochem, Fisher, Sigma.
Chick Plasma [Links, Images, Wikipedia/plasma] Invitrogen/Gibco.
Chicken Serum [Links, Images, Wikipedia/Serum]. Invitrogen/Gibco (Nalge Nunc), ICN.
Chloros [Links, Images] Hays Chemical Distribution.
CHO Cells (Chinese Hamster Ovary Cells) [Links, Images, Video, Wikipedia/CHO cells; Index/Protein Production for Recombinant Proteins; Links/Single Use Bioreactors, Images, Papers, Books; Bioreactors; ActiCHO CHO medium for active cells (up to 5-fold increase in productivity), Links/CHO cell media, Images, Papers, Books].
Chromosome Paints [Links, Images, Video, Wikipedia/chromosome_paints] Applied Imaging, Cambio.
Cloning rings [Links, Images] Bellco, Fisher, Scientific Laboratory Supplies.
Clorox [Links, Images] Polyscience.
Closed-circuit TV [Links, Images] Dage-MTI, Hammamatsu, Leica, see also microscopes.
CMC [Links, Images, Wikipedia] See carboxymethylcellulose.
CO2 automatic change-over unit for cylinders [Links, Images] Gow-mac, Lab Impex, Shandon.
CO2 controllers [Links, Images] Air Products, Forma, IEC.
CO2 incubators [Links, Images] Assab, Forma, Heinecke, Haraeus, ICN, IEC (Hotpak),
___Lab-Line, LEEC, Napco, New Brunswick Scientific, NuAire, Precision Scientific, Sanyo Gallenkamp.
Colcemid [Links, Images] Sigma.
Collagen [Index, Links, Images, Video, Papers, Books, Wikipedia] Boehringer, Becton Dickenson,
___Cambridge Biosciences, Collagen Corporation, Invitrogen/Gibco, ICN, Sigma, Universal Biologicals.
Collagenase [Index, Links, Images, Video, Papers, Books, Wikipedia] Worthington, Sigma, Boehringer.
Colony counter [Links, Images] BioWorld, NEN Life Sciences, Optomax, Perceptive Instruments.
Confocal Microscope [Links, Images, Video, Papers, Books, Wikipedia]
___Bio-Rad, Biotech Instruments, Leica, Molecular Dynamics, Nikon, Zeiss.
Controlled-rate coolers for liquid N2 freezing. [Links, Images].
Coverslips: [Links, Images]
___ glass coverslips: [Links, Images] Bayer, Fisher, Invitrogen/Gibco (Nalge Nunc), Wheaton.
___ plastic coverslips: [Links, Images] Bayer, ICN, Lux, Invitrogen/Gibco (Nalge Nunc).
Crystal Violet [Links, Images] Merck, Fisher.
Cytobuckets [Links, Images] IEC.
Cytocentrifuge [Links, Images]
___CSP, IEC, Miles, Shandon, Wescor.
Cytokines [Index, Links, Images, Video, Papers, Books, Wikipedia]
___See growth factors [Wikipedia].
Cytometer [Links, Images, Video, Papers, Books, Wikipedia/cytometer]
___flow cytometer [Wikipedia/flow cytometer],
___scanning cytometer [Wikipedia/scanning cytometer].
Culture Flasks, dishes, and plates [Links, Images] See tissue culture flasks, ect.
DAEA dextran [Links, Images] Amershan Pharmacia, Bio-Rad.
Deionizers [Links, Images] Bellco, Corning, Elga, Millipore.
Densitometers [Links, Images, Video, Wikipedia]
___Beckman Instruments, Pall Gelman Sciences, Gilford Instruments,
___Helena, Joyce-Lobel, Molecular Dynamics.
Density Meter [Links, Images] Mettler Toledo, Parr.
DPX, Permount See stains.
Detergents [Links, Images, Wikipedia] Alconox, Calbiochem, Decon, ICN, Pierce.
Dexamethasone [Links, Images] Sigma, Merck.
Dextran [Links, Images] Calbiochem, Fisher, Sigma.
Diacetyl fluorescein [Links, Images] Fisher, Sigma.
Dialysis tubing [Links, Images] Laboratory suppliers.
Disc Filter assembly for sterilization [Links, Images] See tissue culture flasks, ect.
Dishes [Links, Images] See tissue culture flasks, ect.
Disinfectants [Links, Images, Video, Wikipedia].
___BioMedical Products, Guest Medical, ICN, Johnson & Johnson,
___Lab Safety Supply, Polysciences, Sigma, Thomas, VWR Scientific.
Dispase [Links, Images] Boehringer Mannheim.
Dispensers, Liquid [Links, Images] Accuramatic, Barnstead, Corning, Gilson, Jencons, Zinsser.
DMSO [Links, Images, Wikipedia] Merck.
DNase [Links, Images] Sigma, Worthington.
DNA fingerprinting [Links, Images, Wikipedia] Cellmark Diagnostics, ECACC.
ECM extracellular matrix [Links, Images, Wikipedia] See matrix.
EDTA [Links, Images, Wikipedia] See chemicals.
Ehrlenmeyer Flasks [Links, Images] See glassware.
Electronic Cell Counter [Links, Images] See cell counters.
Electronic thermometer [Links, Images] Comark, Fisher, Grant, Omega, Rustrak.
Electrophoresis [Links, Images, Wikipedia]
___Amersham Pharmacia, Anachem, Bio-Rad, Gibco/BRL,
___Haake, Innovative Chemistry, Life Sciences International, Shandon.
Emulsion for Autoradiography [Links, Images, Wikipedia] Amershan Pharmacia.
Epidermal Growth Factor [Links, Images, Wikipedia] See growth factors.
Ethidium Bromide [Links, Images] Sigma.
Extracellular matrix [Links, Images] See matrix.
Fermentors [Links, Images, Wikipedia]
___Alfa Laval, Bellco, Biotech Instruments, Braun,
___Cellon, Genetic Research Instrumentation, New Brunswick Scientific.
Fibroblast Growth Factor [Links, Images, Wikipedia]. See growth factors.
Fibronectin [Links, Images, Wikipedia] See growth factors, matrix.
Ficoll [Links, Images] Amersham Pharmacia, ICN.
Ficoll-paque [Links, Images] Amersham Pharmacia, BioWhittaker, ICN, Nycomed.
Filters [Links, Images] Becton Dickinson, Pall Gelman, Millipore, Nalge Nunc, Sartorius.
Filter holders [Links, Images] See filters.
Filter well inserts [Links, Images] Becton Dickenson, Bibby Sterlin, Corning Costar, Integra,
___Millipore, Nalge Nunc. See filters.
Filtration Equipment [Links, Images, Wikipedia] See filters.
Flasks [Links, Images] See tissue culture flasks, ect.
Flow Cytometers [Links, Images, Wikipedia] Becton Dickenson, Beckman Coulter.
Fluorescence-activated cell sorter (FACS) [Links, Images, Wikipedia] Becton Dickenson.
Fluorescence fade retardant [Links, Images] Vector.
Freezers, -20 degrees C [Links, Images, Wikipedia] Barnstead, Fisher,
___New Brunswick Scientific, local discount warehouses.
Freezers, -70 degrees C [Links, Images] Barnstead, ___Fisher,
Life Sciences International, Revco.
Freezers, liquid nitrogen [Links, Images, Wikipedia/liquid_nitrogen]
___Cryo-med, Aire Liquide, MVE,
___Planer, Taylor Wharton, Thermolyne.
Fungicides [Links, Images, Wikipedia/fungicides] BioWhittaker, ICN, Invitrogen/Gibco (Nalge Nunc), Sigma.
Fungizone [Links, Images] See amphotericin B.
Gases [Links, Images].
Gas-permeable caps [Links, Images] Beckton Dickenson, Corning Costar, Nalge Nunc.
Gentamicin [Links, Images] Schering, Invitrogen/Gibco.
Giemsa stain [Links, Images] Fisher, Merck.
Gilson Pipettman [Links, Images] Bellco, Corning, Gilson, Schott, Wheaton.
Glassware [Links, Images, Wikipedia] Bellco, Corning, Schott.
Glassware washing machine [Links, Images]
___Lancer, Miele, Scientek (John Burge in U.K.), Steris Corp., Vernitron (USA).
Glucose [Links, Images, Wikipedia]
___Fisher, Invitrogen/Gibco (Nalge Nunc), ICN, Merck, Sigma.
Glutamine, powder [Links, Images, Wikipedia] Sigma.
Glutamine, 200 mM [Links, Images, Wikipedia] BioWhittaker, ICN, Invitrogen/Gibco (Nalge Nunc), Sigma.
Glutathione [Links, Images, Wikipedia] Sigma.
Glycerol [Links, Images, Wikipedia] Fisher, Merck.
Growth Factors [Links, Images, Wikipedia] ___BioWhittaker, Boehringer, Cambridge Biosciences,
___Collaborative Research (Becton Dickenson), Genzyme, Invitrogen/Gibco,
___ICN, Promega, R&D Sigma, Upstate Biotechnology.
HEK293 Cells [Links, Images, Video, Wikipedia/HEK cells; Index/Protein Production for Recombinant Proteins; Links/Single Use Bioreactors, Images, Papers, Books; Bioreactors]
Hemocytometer [Links, Images, Wikipedia] Fisher, Shandon.
HEPES [Links, Images] ICN, Invitrogen/Gibco, Sigma.
Hoechst 33258 fluorescent mycoplasma stain [Links, Images] Hoechst, ICN (complete kit), Sigma.
Human Cells [Wikipedia/Human cells, Links, Images, Video, Papers, Books;
__Links/Human Cell Cultures, Images, Video, Papers, Books;
__Links/Human Stem Cells, Images, Video, Papers, Books;
__Links/Human Stem Cell Cultures, Images, Video, Papers, Books;
__Links/Human Cancer Cells, Images, Video, Papers, Books;
__Links/Human Cancer Cell Cultures, Images, Video, Papers, Books;
__Links/Human Senescent Cells, Images, Video, Papers, Books;
__Links/Human Cell Protocols, Images, Video, Papers, Books].
Hyaluronidase [Links, Images, Wikipedia] Sigma, Worthington.
Hydrocortisone [Links, Images, Wikipedia] Merck, Sigma.
Hypaque-Ficoll media [Links, Images] Amersham Pharmacia, BioWhittaker, ICN, Nycomed.
Hypochlorite disinfectant [Links, Images] See disinfectants [Wikipedia].
Hypoclearing agent [Links, Images] Kodak.
Hypoxanthine [Links, Images, Wikipedia] Sigma.
Image analysis [Links, Images, Wikipedia]
___Artek, Hamamatsu, Imaging Research, Leica,
___Molecular Dynamics, NEN Life Sciences, Perceptive Instruments, Zeiss.
Incubators [Links, Images, Wikipedia]
___Barnstead, Forma, Heraeus, Lab-Line,
___LEEC, Napco, New Brunswick Scientific,
___Nuaire, Precision Scientific Co.
Instruments, dissecting [Links, Images, Wikipedia] Fisher.
Insulin [Links, Images, Wikipedia] Intergen, Sigma.
Interleukins [Links, Images, Wikipedia] See growth factors [Wikipedia].
Inverted Microscope [Links, Images, Wikipedia] Leica, Nikon, Olympus, Zeiss.
Isotopes [Links, Images, Wikipedia] See radioisotopes.
Kanamycin [Links, Images] Invitrogen/Gibco, Sigma.
Lactalbumin hydrolysate [Links, Images] Difco, Invitrogen/Gibco.
Laminar-flow hoods [Links, Images]
___Atlas Clean Air, Baker, Contamination Control, Envair, Gelman,
___Germfree Labs, Heto-Holten, Labcaire,
___MDH, Medical Air Technology, Nuaire.
Laminin [Links, Images, Wikipedia] Becton Dickinson, Invitrogen/Gibco (Nalge Nunc), Lab Vision, Sigma.
Latex Particles (standard) [Links, Images, Wikipedia] Beckman Coulter.
Liquid N2 freezer [Links, Images, Wikipedia] See freezers, liquid nitrogen.
Lymphocyte preparation media [Links, Images]
___Amersham Pharmacia, BioWhittaker, ICN, Nycomed, Robbins Scientfic.
Magnetic Stirrers [Links, Images]
___Bellco, Camlab, Chemap, Fisher,
___Hanna, Jencons, Lab-Line,
___Techne, Thomas, Wheaton.
Magnifying viewers [Links, Images] Bellco, New Brunswick Scientific.
Marker beads [Links, Images] Amersham Pharmacia.
Matrix [Links, Images] Becton Dickenson, Biofluids, Cellon, Imperial, Pierce, R&D Sigma, TCS.
Media, cell culturing [Links, Images]
___Biofluids, Inc., BioWhittaker, Connaught, ICN, Irvine Scientific,
___Invitrogen/Gibco (Nalge Nunc), JRH Biologicals/Seralab, Paesel, Promocel, Sigma.
Media bags [Links, Images] DuPont, ICN, Sigma.
Mercaptoethanol [Links, Images, Wikipedia] Merck, Fisher, Sigma.
Methocel [Links, Images] Dow Corning.
Methylcellulose [Links, Images, Wikipedia] Dow Corning, Fluka, Fisher, Metachem, Stem Cell Technology.
Metrizamide [Links, Images, Wikipedia] Nycomed.
Microcaps [Links, Images] (Drummond) Shandon, Fisher.
Microcarriers [Links, Images].
___Amersham Pharmacia, Bio-Rad, Nalge Nunc,
___Invitrogen/Gibco, ICN, Sigma, TCS.
Microdensitometry [Links, Images, Wikipedia] See CCD Cameras, scanning cytometer.
Micropipettes [Links, Images] Alpha, Anachem, Applied Scientific, Bibby, Boeringer, Costar, Eppendorf, Gilson.
Microscopes [Links, Images, Wikipedia] Leica, Olympus, Nikon, Zeiss.
Microscopes, fluorescence [Links, Images, Wikipedia] See microscopes.
Microscope Slides [Links, Images]
___Lab-Tek, Bellco, general laboratory suppliers, Nalge Nunc (Invitrogen/Gibco).
Microtitration plates [Links, Images, Wikipedia] See tissue culture, flasks, ect.
Microtitration plates with removable wells [Links, Images] Invitrogen/Gibco (Nalge Nunc).
Microtitration plate sealers [Links, Images] See plate sealers.
Mitomycin C [Links, Images] Sigma.
Multipoint pipettors [Links, Images] See micropipettes and pipettors.
Multiwell plates [Links, Images] See tissue culture, flasks, ect.
Mycostatin, Nystatin [Links, Images] BioWhittaker, ICN, Invitrogen/Gibco, Sigma.
Naphthalene black [Links, Images, Wikipedia] See stains.
Needles (for syringes) [Links, Images] See syringes.
Nucleosides [Links, Images, Wikipedia] Sigma.
Nutrient broths [Links, Images] Difco, Invitrogen/Gibco (Nalge Nunc).
Nylon film [Links, Images] Buck Scientific, Portex, Portland Plastics.
Nylon mesh [Links, Images] Stanier or see cell strainers.
Oncostatin M [Links, Images] See growth factors.
Organ culture grids [Links, Images] Becton Dickenson.
Osmometer [Links, Images, Wikipedia] Advanced Instruments, Clandon Scientific.
Packaging; cartridge paper, semipermiable nylon film [Links, Images]
___Hospital suppliers, Buck Scientific, Portex, Portland Plastics.
PBS A&B [Links, Images] Invitrogen/Gibco (Nalge Nunc), ICN, Oxoid, Sigma.
Penicillin [Links, Images, Wikipedia] BioWhittaker, Invitrogen/Gibco, ICN, Sigma.
Percoll [Links, Images] Amersham Pharmacia.
Perfusion culture [Links, Images] Amicon, Cellco, Endotronics, Microgon.
Petri dishes [Links, Images] See tissue culture flasks, ect.
Petri perm dishes [Links, Images] Heraeus.
Photographic chemicals, films, and papers [Links, Images, Wikipedia/photography]
___Agfa, Eastman Kodak, Fuji, Ilford (and local camera shops).
Phytohemagglutinin [Links, Images] Amersham Pharmacia, Sigma.
Pipettes [Links, Images, Wikipedia] Bellco, A.R.Horwell, Corning, Fisher, Voltac, Sterilin, Becton Dickenson.
Pipetting aids [Links, Images] Alpha, Bellco, Costar, Horwell.
Pipette cans [Links, Images] Bellco, Life Sciences International.
Pipette cylinders [Links, Images] Bel Art, Fisher, Nalge Nunc (Invitrogen/Gibco).
Pipette plugger [Links, Images] Bellco, Volac (John Poulton).
Pipette washer, drier [Links, Images] Bel Art, Shandon, Radleys.
Pipettors [Links, Images]
___Alpha, Anachem, Applied Scientific, Barnstead,
___Bellco, Bibby, Boehringer, Corning Costar,
___Eppendorf, Fisher, Gilson, Integra.
Plasma, equine [Links, Images, Wikipedia] ICN.
Plate readers [Links, Images] Bio-Tek, Bio-Rad, Canberra-Packard, Dynex, Fisher, Molecular Dynamics.
Plates [Links, Images] See tissue culture flasks, ect.
Plate sealers [Links, Images] Anachem, Elkay, ICN.
Pleated cartridge filter [Links, Images] Pall Gelman.
Pluronic F-68 [Links, Images] Invitrogen/Gibco, ICN, Serva, Sigma.
Polaroid Camera [Links, Images] Polaroid.
Poly-D-lysine [Links, Images] Sigma.
Polystyrene flasks [Links, Images] See tissue culture flasks, ect.
Polyvinyl pyrrolidone [Links, Images] Calbiochem, Sigma.
Pressure cooker, bench-top autoclave [Links, Images]
___Astell Scientific, Harvard Apparatus, Inc., LTE,
___Napco, Valley Forge.
Pronase [Links, Images] Sigma.
Propidium iodide [Links, Images] Sigma.
Protective Clothing [Links, Images] Lab Safety Supply, Sigma, Alexandria Workwear.
Pumps, peristaltic [Links, Images, Wikipedia]
___Amersham Pharmacia, Cole Parmer, Gilson, Horwell,
___Millipore, Watson-Marlowe, Zinsser.
Pumps, vacuum [Links, Images, Wikipedia] Millipore, Pall Gelman, BOC.
Quinacrine dihydrocloride [Links, Images] Sigma.
Radioisotopes [Links, Images, Wikipedia]
___Amersham International, ICN, New England Nuclear, (Dupont), Sigma.
Recording thermometer [Links, Images]
___ABB Kent-Taylor, Comark, Cole Parmer, Fisher, Harvard,
___Grant, Omega, Pierce, Rustran.
Refractometer [Links, Images, Wikipedia] Beckman, Cole Parmer, Fisher.
Refrigerators [Links, Images, Wikipedia] Local discount warehouses.
Reusable in-line filter assembly [Links, Images] See filters.
Reverse osmosis [Links, Images, Wikipedia] Barnstead, Elga, Millipore, U.S. Filter.
Roccall Henry Schein Rexodent.
Roller bottles, glass [Links, Images] Bellco.
Roller bottles, plastic [Links, Images] See tissue culture flasks, ect.
Roller bottle rack [Links, Images] Bellco, Integra, New Brunswick Scientific.
Rubber pipette bulb [Links, Images]
___Bel Art, Bellco, Bibby, Fisher, Jencons Scientific, Ltd.
Safety Cabinets [Links, Images] See laminar-flow hoods.
Scalpels [Links, Images, Wikipedia] See instruments, dissecting.
Scanning Cytometer [Links, Images, Wikipedia] Compucyte.
Scintilation Fluid [Links, Images]
___Amersham Pharmacia, BS & S, National Diagnostics, New England Nuclear (Dupont), Packard.
Scintillation vials, minivials [Links, Images] Canberra Packard.
Semipermiable Nylon Film [Links, Images] See packaging.
Serum [Links, Images, Wikipedia]
BioWhittaker, Invitrogen/Gibco (Nalge Nunc), Globepharm, Hyclone,
ICN, JRH Biosciences, Sigma.
Serum substitutes: [Links, Images]
___Biotain-MPS BioWhittaker.
___CPSR Sigma.
___Ex-cyte Bayer.
___Excell-900 JRH Biosciences.
___ITS Premix ICN.
___Nutridoma Boehringer
___Serxtend NEN (Dupont).
___SIT Sigma.
___TCM, TCH ICN.
___Ultroser Invitrogen/Gibco.
___Ventrex JRH Biosciences.
Serum-free media [Links, Images, Wikipedia]
___BioWhittaker, Boehringer, Biofluids, Clonetics,
___Invitrogen/Gibco (Nalge Nunc), Hyclone, ICN, Irvine Scientific,
___JRH Biosciences, Mediacult, Promacell, Sigma.
Sieves [Links, Images] Becton Dickenson, Sigma.
Silica Gel [Links, Images] Fisher, Merck.
Silicones [Links, Images] Fisher, Merck, Sigma.
Silicone Tubing [Links, Images]
___Dow Corning, ESCO, Bibby, Sterilin.
Slide boxes, light tight, [Links, Images] Becton Dickenson, Raven Scientific.
Slide containers for emulsion (Cyto-Mailer) [Links, Images] Lab-Tek, Fisher.
Specialized cell cultures [Links, Images, Wikipedia]
___Clonetics, BioWhittaker, Cell Systems, Promocell.
Stains [Links, Images, Wikipedia] Merck, Fisher.
Stainless steel mesh [Links, Images] Becton Dickenson, Sigma.
Sterility indicators [Links, Images]
___Applied Scientific, Bennett, Jencons,
___Popper, Surgicon.
Sterilization bags [Links, Images] See packaging.
Sterilization film [Links, Images] Seek packaging.
Sterilizing and drying oven [Links, Images]
___Astell Hearson, Cole Parmer, Camlab, Fisher,
___Forma, Harvard, Horwell,
___LEEC, LTE, New Brunswick Scientific, Precision Scientific.
Sterilizing tape (indicator) [Links, Images]
___Applied Scientific, Barnstead, Fisher,
___Roboz-Surgical, Shamrock, 3M (laboratory suppliers).
Stills [Links, Images] Corning, Jencons, Steris.
Stirrers [Links, Images] See magnetic stirrers.
Streptomycin [Links, Images, Wikipedia] Invitrogen/Gibco (Nalge Nunc), Sigma.
Syringes [Links, Images, Wikipedia] Becton Dickenson, Popper, (and local laboratory suppliers).
Temperature Controllers [Links, Images] See thermostats, proportional controllers.
Temperature recorders [Links, Images] See recording thermometer.
Thermalog [Links, Images] Bennet, Popper.
Thermanox [Links, Images] See coverslips, plastic.
Thermostats, proportional controllers [Links, Images, Wikipedia]
___Controls & Automation, Fisher, Napco.
α-Thioglycerol [Links, Images] Sigma.
Thymidine [Links, Images, Wikipedia] Sigma.
Time-lapse video [Links, Images] See CCD cameras, video camera, video recorder.
Tissue culture flasks, ect. [Links, Images, Wikipedia/Tissue_Culture]
___Nalge Nunc (Invitrogen/Gibco), Becton Dickenson, Bibby,
___Corning Costar, Linbro, Lux (ICN).
Tissue culture media [Links, Images] See media.
Trypan blue [Links, Images, Wikipedia] See stains.
Trypsin [Links, Images, Wikipedia]
___ICN, Invitrogen/Gibco, Sigma, Worthington.
Trypsin inhibitor (soya bean) [Links, Images] Sigma.
Tryptophan [Links, Images, Wikipedia] See amino acids.
Tryptose phosphate broth [Links, Images] Difco, Invitrogen/Gibco, Oxoid.
Tyrosine [Links, Images, Wikipedia] See amino acids.
Universal Containers [Links, Images] Sterilin, Nalge Nunc (Invitrogen/Gibco).
Vacuum Pump [Links, Images] See pumps.
Video camera [Movie Making, Links, Images; Links/for cellular microscopy, Images]
___See CCD cameras, microscopes.
Video Recorder [Links, Images] Hamamatsu.
Vinblastin [Links, Images] Sigma.
Vinyl Tape [Links, Images] 3M (laboratory suppliers).
Vitamins, solid [Links, Images; Links/for cell biolabs, Images]
___Sigma.
Vitamins, solution [Links, Images; Links/for cell biolabs, Images]. See media.
Vortex mixer [Links, Images] Gallenkap, Fisher, general laboratory suppliers.
Water purification [Links, Images; Links/lab water purifiers, Images].
___Barnstead, Elga, Millipore, U.S.Filter.
X-ray film [Links, Images, Papers, Patents, Books; Links/X-ray film sources]
___Eastman Kodak Company, Fuji.

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Disclaimer
These are notes of an MSEE and student of life extension technique. The intent is to construct an expert system pertaining to the lab science associated with anti-aging medicine and related disciplines. For in-person expert advice, consult your physician and health professionals. They can usually do the appropriate laboratory tests to properly diagnose your condition. Note that many of the methods of molecular biology we might specify to test a hypothesis involve chemicals that are neurotoxic or carcinogenic, or methods that are dangerous in other ways. You should avoid doing dangerous experiments without appropriate laboratory safety measures and suitable professional guidance. Also, sometimes unstated rules apply to biomethod protocols, such as a requirement to maintain a constant gel temperature, that can spoil a result. Be sure to wear a face mask around cell lysis buffers and other bioscience chemicals; at least one of them can equip you with a triangle of mole marks around your mouth resembling Darth Vader's helmet.

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